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DLL3 antibody

Anti-DLL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03439
Anti-DLL3 antibody used in Western Blot (WB). GTX03439
Anti-DLL3 antibody used in Western Blot (WB). GTX03439
Anti-DLL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03439
Anti-DLL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03439
Anti-DLL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03439
Anti-DLL3 antibody used in Western Blot (WB). GTX03439

Cat. No. GTX03439

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
200 μl ($399)
See all DLL3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:25
IHC-P 1:500
Not tested in other applications.

Calculated MW

65 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 519-548 amino acids from the C-terminal region of human DLL3.

Purification

Protein A purified followed by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

delta like canonical Notch ligand 3 , SCDO1

Cellular Localization

Membrane

Background

This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DLL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03439

GTX03439 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed U2OS cells using GTX03439 DLL3 antibody.
Green : Primary antibody
Red : Actin
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:25

Anti-DLL3 antibody used in Western Blot (WB). GTX03439

GTX03439 WB Image

WB analysis of various sample lysates using GTX03439 DLL3 antibody.
Lane 1 : A375 whole cell lysate
Lane 2 : U-251 MG whole cell lysate
Lane 3 : NCI-H1299 whole cell lysate
Lane 4 : Human brain tissue lysate
Lane 5 : Mouse brain tissue lysate
Lane 6 : Rat brain tissue lysate
Dilution : 1:1000
Loading : 20 μg per lane

Anti-DLL3 antibody used in Western Blot (WB). GTX03439

GTX03439 WB Image

WB analysis of various sample lysates using GTX03439 DLL3 antibody.
Lane 1 : NCI-H1299 whole cell lysate
Lane 2 : Human brain tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Rat brain tissue lysate
Dilution : 1:1000
Loading : 20 μg per lane

Anti-DLL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03439

GTX03439 IHC-P Image

IHC-P analysis of human testis tissue using GTX03439 DLL3 antibody.
Antigen retrieval : Heat mediation with a EDTA buffer (pH9.0)
Dilution : 1:500

Anti-DLL3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03439

GTX03439 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed U2OS cells using GTX03439 DLL3 antibody.
Green : Primary antibody
Red : Actin
Permeabilization : 0.1% Triton X-100
Dilution : 1:25

Anti-DLL3 antibody used in IHC (Paraffin sections) (IHC-P). GTX03439

GTX03439 IHC-P Image

IHC-P analysis of human brain tissue using GTX03439 DLL3 antibody.
Antigen retrieval : Heat mediation with a EDTA buffer (pH9.0)
Dilution : 1:500

Anti-DLL3 antibody used in Western Blot (WB). GTX03439

GTX03439 WB Image

WB analysis of various sample lysates using GTX03439 DLL3 antibody.
Lane 1: A375 whole cell lysate
Lane 2 : human brain tissue lysate
Lane 3 : human liver tissue lysate
Dilution : 1:2000
Loading : 20 μg per lane

REFERENCE

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Package List Price ($)
$ 399